The Animal Health Laboratory provides a front-line
service for the isolation and culture of salmonid viruses enzootic to Tasmania.
Where there is a suspicion of a new or exotic virus, AHL will refer samples for
testing to the Australian Animal Health Laboratory, CSIRO, Geelong.
Cell lines available
Isolation of viruses can be undertaken on up to five
fish cell lines: CHSE-214, ASK, EPC, BF2 and RTG-2. The number and type of cell
lines used is dependent on the type of investigation required. Before submitting
samples, please contact Fish Virology to discuss the most appropriate cell
lines to be used.
Currently, salmonid viruses, which can be routinely
isolated and identified include: Tasmanian aquabirnavirus; Tasmanian
aquareovirus and pilchard orthomyxovirus
Before sending in samples for virus culture, first
contact the Animal Health Laboratory to make a booking. We can advise on the
type and number of cell lines which should be used for the investigation and
the most appropriate means of sample collection and submission. Although it
will take at least 7 days for the cell lines to be made ready, samples can be
submitted immediately after booking them in and will be held by the laboratory
until they can be inoculated on to the cell lines.
Submission can either be as whole-fish on ice or as
tissues collected at a field station or base laboratory. General guidance on
sample collection can be found in the
Guidelines and Procedures Manual for Field Sampling of Fish for Disease Investigation and Health Monitoring (625Kb)
on collecting virology samples can be found in the
AHL LabFact Sampling for Virology testing (204Kb)
Growth of salmonid viruses is slow. At minimum it will
take at least three weeks for viruses to appear and be identified. AHL will
provide interim results as appropriate.
Contact Fish Virology
T: 03 6777 2247
pre-smolt Atlantic salmon in a hatchery tank
copyright: Scott Godwin